Immunocorrective potential of peptidoglycan monomer linked with zinc (PGM-Zn) in experimental jaundice (CROSBI ID 464929)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Ravlić-Gulan, Jagoda ; Ćuk, Mira ; Radošević-Stašić, Biserka ; Petković, Marija ; Dvornik, Štefica ; Rukavina, Daniel
engleski
Immunocorrective potential of peptidoglycan monomer linked with zinc (PGM-Zn) in experimental jaundice
Introduction: Patients with jaundice due to extrahepatic biliary obstruction still experience a high rate of postoperative complications and death. High levels of bile salts and intracellular bilirubin are suspected as mediators of the systemic consequences of obstructive jaundice, but recent data suggest that disturbances in macrophage-immune system interactions may be responsible.Materials and Methods: This study focused on the immunological, phagocytic and biochemical alterations of BALB/c mice subjected to common bile duct ligation (CBDL) and potential therapeutic effects of PGM-Zn, which in our previous experiments has shown immunostimulatory and hepatotropic effects. Treatment with PGM-Zn (10 mg/kg i.p.) or saline solution started 6 days before CBDL. Immune response disturbances were analysed in three postoperative days by estimation of phenotype and cell cycle of cells in thymus and spleen. We also measured phagocytic capacity of peritoneal macrophages and class II MHC expression in the liver. Biochemical alterations were followed by measuring the serum concentration of AP, GGT, ALT, AST, cholesterol, albumins and total proteins.Results: The data have shown that on the first p.o. day in thymus increases the number of CD4+CD8- and CD4- CD8+ cells and decreases the proportion of double positive cells. Subsequently, the number of double negative cells increased. In the spleen during the cholestasis a decrease of proportion of CD4+, CD5+ and Ig+ cells were also seen. The effects were followed by increased number of cells in S and G2/M phases. Pre-treatment with PGM-Zn prolonged the survival of mice with CBDL. Concomitantly, expression of class II in the liver increased, and augmented the proportion of double negative and CD4+CD8- cells in thymus, and CD4+ and Ig+ cells in spleen. Hyperplastic effect of PGM-Zn on thymic and splenic cells was proved by cell cycle analysis. PGM-Zn had also some influences on biochemical alterations characteristic for obstructive jaundice. Conclusion:The data point to CBDL-induced immunosuppression and to therapeutic potential PGM-Zn in these events.
cholestatic jaundice; peptydoglycan-monomer linked with zinc; immunosuppression
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Podaci o prilogu
83-83-x.
1997.
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objavljeno
Podaci o matičnoj publikaciji
Immunology Letters (Abstract Issue) 56 (1997)
Brent, L.
Amsterdam: Elsevier
Podaci o skupu
Fourth International Symposium on Clinical Immunology
poster
19.06.1997-21.06.1997
Amsterdam, Nizozemska