Expression of bone morphogenetic proteins in long-term culture of human bone marrow stromal cells (CROSBI ID 481066)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Kisić, Veronika ; Mazić, Sanja ; Bašić, Nikolina ; Martinović, Snježana ; Batinić, Drago ; Labar, Boris ; Vukičević, Slobodan
engleski
Expression of bone morphogenetic proteins in long-term culture of human bone marrow stromal cells
BMP are expressed in bone marrow stromal cells and involved in regulation oh hematopoiesis. The aim of this investigation was to explore expression of bone morphogenetic proteins, their receptors and BMP second messengers in long- term culture of human bone marrow stromal cells. Hematopoietic activity was present throughout the investigation period. RNA was isolated from cultivated cells using a TRIzol reagent ; cDNA was then synthesized and RT-PCR performed using primers for BMP-2 to 7, ALK-2, 3 and 6 and Smad-1, 4 and 5. The results indicate that bone marrow stromal cells produce mRNA for ALK-2 and 6, Smad-1, 4 and 5, and both mRNA and protein BMP-3, 4 and 7. Since hematopoietic cells have BMP receptors, a paracrine mechanism in regulating hematopoiesis is suggested.
BMP; marrow stromal cells; hematopoiesis; long- term culture of human bone marrow stromal cells
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Podaci o prilogu
92-92.
2000.
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objavljeno
Podaci o matičnoj publikaciji
Calcified tissue international. Supplement
0944-0747
Podaci o skupu
European Symposium on Calcified Tissue (27 ; 2000)
poster
05.05.2000-10.05.2000
Tampere, Finska