Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro (CROSBI ID 206524)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Galić, Elizabeta ; Tadin, Antonija ; Galić, Nada ; Kašuba, Vilena ; Mladinić, Marin ; Rozgaj, Ružica ; Biočina-Lukenda, Dolores ; Galić, Ivan ; Želježić, Davor
engleski
Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro
The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unpolymerised, was eluted in extracellular saline (1 cm2 mL-1) for 1 h, 1 day, and 5 days. Cultures were treated with eluates using final dilutions of 10-2, 10-3, and 10-4. Alkaline comet assay did not detect changes in DNA migration of treated cells regardless of the ionomer tested, polymerisation state, and elution duration. Glass ionomers failed to significantly influence micronucleus frequency. No oxidative DNA damage in treated lymphocytes was observed using hOGG1 modified comet assay. Obtained results indicate high biocompatibility of all tested materials used in the study under experimental conditions.
biocompatibility ; cytotoxicity ; DNA damage ; genotoxicity ; lymphocytes
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Podaci o izdanju
65 (2)
2014.
179-187
objavljeno
0004-1254
1848-6312
10.2478/10004-1254-65-2014-2392