engleski

The antimicrobial effectiveness of photodynamic therapy used as an adjunct to conventional endodontic re-treatment : a clinical study

The purpose of the study was to evaluate the efficacy of antimicrobial photodynamic therapy (aPDT) used as an adjunct to the endodontic re- treatment in the eradication of microorganisms from previously filled root canals. The study sample consisted of 21 randomly selected patients with root filled and infected root canal system with chronic apical periodontitis on incisors or canines, who have had previously endodontic treatment, more than two years ago. Microbiological samples from the root canals were collected after accessing the canal, following the endodontic re-treatment and after the aPDT procedure. During instrumentation, the root canals were irrigated with 2, 5% sodium hypochlorite (NaOCl), and the final irrigation protocol included 17% ethylenediaminetetraacetic acid followed by NaOCl and saline solution. Root canals were filled with a photosensitizing agent and irradiated with a diode laser (100 mW) for 1 min. Microbiological samples from the root canals were cultivated, and the identification was done by micromorphology, macromorphology and different API strips as well as bacterial counts (colony forming units). Fourteen bacteria species were isolated from the root canals, with a mean value of 4.57 species per canal. There was statistically significant reduction in the number of bacteria species and bacterial counts per canal after each step of the root canal treatment (p<0.001). In eleven teeth there were no growth of CFUs after the combination of the chemomechanical preparation and the aPDT. The results indicated that the aPDT used as an adjunct to the conventional chemomechanical preparation achieved a significant futher reduction of intracanal microbial load.

root canal; disinfection; photodynamic therapy

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