engleski

Identification, localization and quantification of porcine intestinal immune cell subsets during the first seven weeks of postnatal ontogeny

By using immunohistology and digital image analysis for histomorphometry we have investigated the influence of porcine postnatal development on distribution and quantitative patterns of naïve/memory lymphoid cell subsets (CD45RA+, CD45RC+, respectively), helper/cytotoxic T cell subsets (CD4+, CD8+, respectively) as well as of IgA+ plasma cells in the gut-associated lymphoid tissues (GALT) of the pigs reared under intensive conditions during the first 7 weeks of life. Current research on postnatal ontogenesis/maturation of porcine small intestinal mucosal immune system have shown that the newborn pigs were immunologically rather immature and, thus, totally dependent on passively acquired immunity. Their jejunal mucosa almost lack the immune cells, with exception of a low number of CD45RA+ lymphoid cells (1.25 x 10-5) at Day 0 post partum. A very few CD45RC+ lymphoid cells (8.79 x 10-6 ) appeared at Day 7 followed by a similar number of IgA+ plasma cells (7.65 x 10-6) which were observed at Day 14. Such first signs of postnatal development of both innate and adaptive immunity by identification/localization of tested cell subsets residing porcine GALT, i e. jejunal mucosal sites are proceeded with a lag period of three weeks until appearance of low numbers of CD4+ (3.62 x 10-5) and CD8+ (3.13 x 10-5) T cells at Day 35 after birth. After that phase of an early postnatal development of the crucial cell subsets participating in the antigen-specific immunity at the gut mucosal surfaces of young pigs, further progress is visible through their different distribution patterns within jejunal mucosa compartments and gradual increase in their numbers particularly between Day 35 and Day 49 of age. A significant increase was recorded within that period for the numbers of naïve, memory and plasma cells whereas the numbers of helper and cytotoxic T cells were only slightly increased between Day 42 and Day 49 of life

postnatal development; immune cells; gut mucosa; pigs.

Rad je vezan uz istraživanja VIP projekta:2012-11-17 naziva: MODULACIJA IMUNOSTI ZDRAVLJA KAO ALTERNATIVA ANTIBIOTICIMA U UZGOJU KONZUMNIH ŽIVOTINJA voditeljice prof. dr. sc. Maje Popović