ADH-catalysed hexanol oxidation with fully integrated NADH regeneration performed in microreactors connected in series (CROSBI ID 207802)
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Šalić, Anita ; Zelić, Bruno
engleski
ADH-catalysed hexanol oxidation with fully integrated NADH regeneration performed in microreactors connected in series
Hexanal is produced by oxidation of hexanol using NADH dependent alcohol dehydrogenase (ADH). Coenzyme, NADH regeneration is needed in order to make ADH catalysed process more sustainable. In this investigation coenzyme regeneration was catalysed by the same enzyme, ADH, which catalysed the main reaction – oxidation of hexanol. Different sources of ADH were studied (suspended and immobilized enzyme ADH and permeabilized baker’s yeast cells) to find optimal catalyst. The best results were obtained by using suspended enzyme where 100% conversion of coenzyme was achieved for very short residence time (τ = 0.8 s). Final phase of investigation was development of integrated system where two microreactor chips were connected in series. First chip was used for hexanol oxidation and the second one for the simultaneous coenzyme regeneration. Regenerated coenzyme was reused by recirculation into first chip where oxidation step was continuously performed for 3 days without the need for addition of fresh coenzyme.
integrated system ; biocatalysis ; hexanol oxidation ; coenzyme regeneration
This study was financially supported by the University of Zagreb short-term financial scientific research support under the title: “Application of Chemical Engineering Methodology in Biocatalytic Process Development”.
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