Study of enzymatic esterification in a solvent system with and without adsorptive control of water with molecular sieves (CROSBI ID 481416)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Giacometti, Jasminka ; Milin, Čedomila ; Vasić-Rački, Đurđa ; Giacometti, Fabio
engleski
Study of enzymatic esterification in a solvent system with and without adsorptive control of water with molecular sieves
Enzymatic esterification of glycerol with oleic acid was carried out in equimolar ratio and catalyzed by immobilized Mucor miehei lipase in a solvent system. The effects of organic solvents, versus log P value, molecular sieves and temperatures were investigated. Lipase catalyzed esterification of glycerol with oleic acid in a solvent system was carried out with 6.67 mg/ml immobilized Mucor miehei lipase at 25, 37 and 50oC, using n-hexane, cyclohexane and isooctane, without and by adding 40 mg/ml of 5Ĺ molecular sieves at the start of esterification, at 100 rpm, in a batch stirrer-tank reactor (BSTR). The reactions were followed by the determination of reaction conversions during 45 hrs. The assay of enzyme reaction was performed by measurement with the pH-stat method and simultaneously monitored by the determination of free oleic acid, monoolein and diolein by gas chromatography method . The highest reaction rates were obtained in nonpolar tested solvents. Changing the system hydrophobicity, by using tested organic solvents, influenced the initial rate conversion but had no affect on the final equilibrium conversion. Changing the tested reaction temperature in the system with molecular sieves, major changes on the initial rate conversion were found at 25oC, but had no affect on the final equilibrium conversion. After 28 h no reaction products were observed with lipase from Pseudomonas cepacia (PS-C and PS-D). The comparison of the used reaction conditions showed the optimal lipase activity in the presence of n-hexane and molecular sieves at 37oC.
enzyme-catalyzed esterification; adsorptive control of water; molecular sieves; solvent system; Lipozym
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Podaci o prilogu
213-213.
2001.
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objavljeno
Podaci o matičnoj publikaciji
10th European Congress on Biotechnology "Biotechnological Challenges in the new Millennium" : abstracts
Perez Mellado, Rafael
Madrid: Sebiot
Podaci o skupu
European Congress on Biotechnology "Biotechnological Challenges in the new Millennium" (10 ; 2001)
poster
08.07.2001-11.07.2001
Madrid, Španjolska