engleski

Histomorphology of the ectopicaly induced bone morphogenesis

Aim: Investigate a dynamics of histogenetic processes of the ectopis osteogenesis. Materials and methods: Demineralized bone matrix implants were implanted subcutaneously pectoral region in rats. Animals were sacrificed after 5, 7, 14, 21 and 30 days of implantation. Hemalun-eosin, toluidine blue and Goldner trichrome staining were obtained. Results: At the 5th day of the implantation, a firm connective fibrous membrane on the surface of the implants was detected. Surface of the demineralized bone particles within the implant is subjected to multinuclear cells degradation, observed as lacunae and fissures. In the implants at the 7th day of the experimental protocol, similar morphological features to those of the 5th day were characterized by: thick fibrous membrane at the surface, high cellular infiltration between bone particles, numerous blood vessels and increased resorption. At the 14th day the first signs of the induced chondrogenesis are seen. Chondrocytic nests, consisted of hypertrophic chondrocytes, surrounded with the small amount of the metachromatic extracellular matrix are situated in between the bone particles. Also, cellular infiltration, bone particle degradation and blood vessels penetration are seen. At the day 21, some cartilage nests are degradated. Newly formed bone trabeculae, built of immature woven bone tissue are seen on the surface of the implant. Between bone particles, three- dimensional network of the bridging bone trabeculae is seen. At the 30th day of the experimental procedure for complete degradation of the implants is present. Conclusion: Histomorphological changes in implants correspond with process of endochondral and intramembranous ossification.

Histomorphology; ectopicaly induced bone morphogenesis

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