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Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation (CROSBI ID 209352)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Coelho, Miguel ; Lade, Steven ; Alberti, Simon ; Gross, Thilo ; Tolić, Iva Marija Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation // PLoS biology, 12 (2014), 6; e1001886, 11. doi: 10.1371/journal.pbio.1001886

Podaci o odgovornosti

Coelho, Miguel ; Lade, Steven ; Alberti, Simon ; Gross, Thilo ; Tolić, Iva Marija

engleski

Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation

Asymmetric segregation of damaged proteins at cell division generates a cell that retains damage and a clean cell that supports population survival. In cells that divide asymmetrically, such as Saccharomyces cerevisiae, segregation of damaged proteins is achieved by retention and active transport. We have previously shown that in the symmetrically dividing Schizosaccharomyces pombe there is a transition between symmetric and asymmetric segregation of damaged proteins. Yet how this transition and generation of damage-free cells are achieved remained unknown. Here, by combining in vivo imaging of Hsp104-associated aggregates, a form of damage, with mathematical modeling, we find that fusion of protein aggregates facilitates asymmetric segregation. Our model predicts that, after stress, the increased number of aggregates fuse into a single large unit, which is inherited asymmetrically by one daughter cell, whereas the other one is born clean. We experimentally confirmed that fusion increases segregation asymmetry, for a range of stresses, and identified Hsp16 as a fusion factor. Our work shows that fusion of protein aggregates promotes the formation of damage-free cells. Fusion of cellular factors may represent a general mechanism for their asymmetric segregation at division.

heat-shock-protein ; fission yeast ; misfolded proteins ; Schizosaccharomyces-pombe ; Saccharomyces-cerevisiae ; Chaperone HSP104 ; quality-control ; budding yeast ; cells ; stress

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Podaci o izdanju

12 (6)

2014.

e1001886

11

objavljeno

1545-7885

10.1371/journal.pbio.1001886

Povezanost rada

Biologija

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