engleski

Genotoxic effects of ZnO nanoparticles im male Wistar rats

Zinc oxide nanoparticles (ZnO NPs) find applications in industry and medicine. The use of nanomaterials has led to the growing concern about their toxicity. Present in vivo study in 10 weeks old male Wistar rats was aimed at investigating the oral toxicty of nano sized ZnO (<35 nm). The genotoxic potential was determined using the alkaline comet assay on kidney, liver and brain cells, and blood lymphocytes. The permanent DNA damage was assessed using the in vivo MN asay in blood. Rats were administered with ZnONPs water dispersion at 5, 10 and 50 mgkg-1bw- 1 daily, or water only for 28 consecutive days through oral gavage. Ethyl methanesulphonate (EMS) as a positive control was given in three daily doses of 300 mgkg-1bw-1 begining at 25.day. For statistical analysis ANOVA and Kruskal-Wallis test were applied. Kidney and brain showed significantly higher values of Tail Intensity (TI) at concentrations of 5 and 50 mgkg-1bw-1 as compared to control. Blood sample significantly differed in TI at a concentration of 50 mgkg-1 compared to all other concentrations including control. All applied concentrations of ZnO NPs in liver significantly increased TI compared to control. MN in vivo test in both erythrocytes and reticulocytes showed significant differences between the highest ZnO NPs concentration and all other applied concentrations. Further in vivo studies with the implementation of new biomarkers should validate these results.

zinc oxide; genotoxicity; nanoparticles; in vivo

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