Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Effects of TSH and calcitriol on bone metabolism: in vivo and in vitro study (CROSBI ID 615788)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Dumić-Čule, Ivo ; Rogić, Dunja ; Ježek, Damir ; Grgurević, Lovorka ; Vukičević, Slobodan Effects of TSH and calcitriol on bone metabolism: in vivo and in vitro study // Bone abstracts. 2014

Podaci o odgovornosti

Dumić-Čule, Ivo ; Rogić, Dunja ; Ježek, Damir ; Grgurević, Lovorka ; Vukičević, Slobodan

engleski

Effects of TSH and calcitriol on bone metabolism: in vivo and in vitro study

Thyroid-stimulating hormone (TSH) exerts both antiresorptive and anabolic effects on bone remodeling in aged ovariectomized rats and TSH -/- mice, supported by clinical results demonstrating that low TSH level is associated with increased bone loss. To further explore the effect of TSH on bone metabolism we here introduced a rat model with removed thyroid and parathyroid glands to obtain low serum concentrations of thyroid and parathyroid hormone, calcitonin and 1, 25(OH)2D3 (approval for the experiment was obtained from ethical committee of University of Zagreb). Surgery resulted in hypocalcemia, low parathyroid and thyroid hormone, 1, 25(OH)2D3, C-telopeptide and osteocalcin serum level. Intermittent administration of TSH resulted in a further decrease of serum calcium and decreased level of serum C-telopeptide due to the suppression of bone resorption, while in the same animals osteocalcin in serum was higher indicating an increased bone formation rate. MicroCT analyses of the distal femur and proximal tibia showed that rats treated with 1, 25(OH)2D3 alone or in a combination with TSH had an increased trabecular bone volume, and enhanced trabecular bone quality. Biomechanical testing of the trabecular bone showed an increased maximal load for 105% and 235%, respectively, in rats treated with 1, 25(OH)2D3 alone, or in a combination with TSH. Rats treated with TSH had a significantly decreased number of osteoclasts in comparison to TPTx control animals. The decline of the osteoclast number was even greater in rats treated with a combination of TSH and 1, 25(OH)2D3, despite the fact that 1, 25(OH)2D3 increased the number of osteoclasts. Addition of TSH to osteoblasts increased the production of the bone specific alkaline phosphatase and had a synergistic effect when combined with 1, 25(OH)2D3. We suggest that TSH independently of calciotropic hormones suppressed bone resorption and stimulated bone formation, while in combination with 1, 25(OH)2D3 acted synergistically on bone formation resulting in an increased bone volume.

thyroid-stimulating hormone; microCT; bone formation

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

2014.

objavljeno

Podaci o matičnoj publikaciji

Bone abstracts

2052-1219

Podaci o skupu

41. European Symposium on Calcified Tissues

poster

17.05.2014-20.05.2014

Prag, Češka Republika

Povezanost rada

Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija