engleski

Novel rat model for osteoporosis research

Osteoporosis is one of the most common metabolic diseases of bones. The field of research on osteoporosis has grown in recent years and numerous potential drugs were tested. For better understanding of drug efficacy and molecular mechanism on bone metabolism there is a great need for establishing appropriate animal model without influence of hormones that affect bone remodeling. Experiments were carried out in male Sprague-Dawley rats, weighting approximately 350-380g. Removal of the thyroid and parathyroid glands (thyroparathyroidectomy - TPTx) was done surgically by a ventral approach under general anesthesia. TPTx resulted in a hypocalcemia with significantly lower serum calcium levels as compared to sham operated control rats. TPTx further resulted in a decreased C-telopeptide and osteocalcin serum level as compared to intact rats. TPTx animals had decreased thyroid hormone and PTH serum level. 1, 25-D(3) was at day 7 decreased about 10 times while TSH was increased 4 to 5 times due to the lack of T3/T4. The effect of TPTx on the trabecular bone was assessed by microCT and biomechanical tests, showing a significantly decreased bone volume and loss of biomechanical strength. We established TPTx rat model and developed conditions for testing the direct influence of several drugs on bone in the absence of calciotropic hormones. After one week rats had a significant trabecular bone loss and they were ready for experimental research.

osteoporosis; bone research; thyroparathyroidectomy

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