engleski

The genetic dependence of RecBCD-Gam mediated double strand end repair in Escherichia coli

The repair of double strand breaks after γ- irradiation in wild-type Escherichia coli lysogenic for λ cI857 red3 is more efficient when λ Gam protein is present. This phenomenon, called gam dependent radioresistance, requires the interaction of RecBCD enzyme and Gam protein. We compared cell survival after γ-irradiation in wild-type and mutant lysogens with and without induction of Gam by transient heat treatment of the cells (6 min, 42°C). The main conclusions are: (1) the RecBCD-Gam pathway of recombination repair is similar but not equivalent to RecBCD‡, a pathway operating in recD mutants ; (2) the RecBCD- Gam pathway is dependent on recJ, recQ and recN gene products and it is proposed that the RecBCD- Gam complex has ability to load RecA protein onto single strand DNA.

escherichia coli ; phage lambda ; RecBCD enzyme ; lambda-Gam protein ; recombination ; repair

nije evidentirano