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izvor podataka: crosbi

Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels (CROSBI ID 213549)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Pletikapić, Galja ; Lannon, Herbert ; Murvai, Unige ; Kellermayer, Mikloš ; Svetličić, Vesna ; Brujić, Jasna Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels // Biophysical journal, 107 (2014), 2; 355-364. doi: 10.1016/j.bpj.2014.04.065

Podaci o odgovornosti

Pletikapić, Galja ; Lannon, Herbert ; Murvai, Unige ; Kellermayer, Mikloš ; Svetličić, Vesna ; Brujić, Jasna

engleski

Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels

Marine-gel biopolymers were recently visualized at the molecular level using atomic force microscopy (AFM) to reveal fine fibril-forming networks with low to high degrees of cross-linking. In this work, we use force spectroscopy to quantify the intra- and intermolecular forces within the marine-gel network. Combining force measurements, AFM imaging, and the known chemical composition of marine gels allows us to identify the microscopic origins of distinct mechanical responses. At the single-fibril level, we uncover force-extension curves that resemble those of individual polysaccharide fibrils. They exhibit entropic elasticity followed by extensions associated with chair-to-boat transitions specific to the type of polysaccharide at high forces. Surprisingly, a low degree of cross-linking leads to sawtooth patterns that we attribute to the unraveling of polysaccharide entanglements. At a high degree of cross-linking, we observe force plateaus that arise from unzipping, as well as unwinding, of helical bundles. Finally, the complex 3D network structure gives rise to force staircases of increasing height that correspond to the hierarchical peeling of fibrils away from the junction zones. In addition, we show that these diverse mechanical responses also arise in reconstituted polysaccharide gels, which highlights their dominant role in the mechanical architecture of marine gels.

marine gel; polysaccharides; self-assembly; force spectroscopy; AFM

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Podaci o izdanju

107 (2)

2014.

355-364

objavljeno

0006-3495

10.1016/j.bpj.2014.04.065

Povezanost rada

Fizika, Geologija, Biologija

Poveznice
Indeksiranost