Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter (CROSBI ID 93467)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Ahel, Ivan ; Vujaklija, Dušica ; Mikoč, Andreja ; Gamulin, Vera Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter // FEMS microbiology letters, 209 (2002), 1; 129-133. doi: 10.1111/j.1574-6968.2002.tb11121.x

Podaci o odgovornosti

Ahel, Ivan ; Vujaklija, Dušica ; Mikoč, Andreja ; Gamulin, Vera

engleski

Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter

Using primer-extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus. A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC-N4-ATTC. However, the major start site of transcription is a G at position −36 and this shorter transcript significantly increases in response to DNA damage by UV-light. The −35 box (TTGTCA) and −10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and this proximal promoter is almost identical to the strong, DNA damage-inducible promoter of Mycobacterium tuberculosis recA gene. We inspected the Streptomyces coelicolor database and found this type of promoter in the upstream regions of many (potentially) UV-inducible genes as well as some other genes/ORFs. Moreover, the DNA sequence between the predicted −35 and −10 boxes is also partially conserved. The consensus sequence for this new type of promoter in Streptomyces is: TTGTCAGTGGC-N6-TAGggT.

recA gene ; Promoter ; Transcription ; UV-induction ; Streptomyces rimosus

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

209 (1)

2002.

129-133

objavljeno

0378-1097

1574-6968

10.1111/j.1574-6968.2002.tb11121.x

Povezanost rada

Biologija

Poveznice
Indeksiranost