engleski

EXTRACELLULAR ENRICHMENT OF SPECIFIC NONVIRION MCMV PROTEINS

Cells have the ability to secrete various membrane vesicles into extracellular environment either by shedding them from plasma membrane or by releasing endosome-derived microvesicles from the intracellular multivesicular body.The secreted microvesicles can deliver proteins and/or nucleic acids to other cells and influence diverse biologic processes including cell proliferation, apoptosis, gene expression and immune regulation. Viral infections can change composition of secreted microvesicles by targeting macromolecules of both cellular and viral origin for secretion. Depending on the viral infection extracellular microvesicles can act either in favour or against maintenance as well spread of viral infection. Microvesicles secreted from infected cells were already described for human cytomegalovirus (CMV), but not for mouse CMV (MCMV). Here we show that MCMV infection of primary mouse embryonic fibroblasts results in enrichment of cellular CD63 protein in the conditioned cell medium. This transmembrane protein is marker of a specific class of extracellular microvesicles named exosomes. In addition, the isolated fraction of conditioned medium contained several nonvirion viral proteins including m04, m06 and IE, but not m152 nor E1. None of the tested proteins associated with MCMV virions, including m57, m55 and m45, were present in the isolated fraction of the conditioned medium.

microvesicles; exosomes; cytomegalovirus

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