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QUERCETIN VS CHRYSIN: EFFECT ON LIVER HISTOPATHOLOGY IN DIABETIC MICE

The antioxidant and reducing ability of quercetin and chrysin and their ability to chelate Fe2+ ions in vitro were studied and compared with their effects on lipid peroxidation and histopathological changes in liver of diabetic mice. Diabetes was induced in Swiss albino mice with a single intravenous injection of alloxan (75 mg kg-1). Two days after alloxan injection, flavonoids preparations (50 mg kg-1 per day) were given intraperitoneally for 7 days in diabetic mice. Antioxidant activity was analyzed as DPPH free radical scavenging activity, reducing power and the capability to chelate iron (II) ions. In vitro chrysin demonstrated notable antiradical activity albeit much lower than quercetin which was an extremely effective radical scavenger. The reducing power of both flavonoids was notable but lower than the activity of ascorbic acid. Both flavonoids demonstrated notable chelating ability towards ferrous ion, chrysin being more active of the two. The lipid peroxidation was evaluated by measuring the MDA production using the 2-thiobarbituric acid TBA test. Administration of quercetin and chrysin to diabetic mice resulted in a significant decrease of lipid peroxidation level in liver tissue. Treatment of diabetic mice with flavonoids solutions results in decreased number of vacuolated cells and degree of vacuolization of the liver tissue. Quercetin and chrysin have beneficial effects on liver histopathology in diabetic mice, according to their antioxidant capacity in vitro. The protective role of flavonoids against the ROS induced damages in diabetic mice gives a hope that they may exert similar protective action in humans.

diabetes; quercetin; chrysin; lipid peroxidation; histopathology

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