engleski

The influence of a palindromic insertion on plasmid integration in yeast

Palindromic sequences act as potential initiators of mitotic and meiotic recombination in yeast. We investigated the influence of a 102 base pair (bp) palindromic insertion present on a non-replicative plasmid on recombination with the yeast chromosome. First we show that the frequency of transformation was not significantly affected by the presence of the perfect palindrome in the CYC1 region. However, as revealed by molecular analysis of transformants, both the proportion and the distribution of recombination events involving chromosomal homology was changed. Our results indicate that the palindrome present on the plasmid molecule was not efficiently processed in vivo to a double-strand break (DSB). We conclude that, in the absence of DNA replication, palindromic sequences are not potent initiators of recombination, but may influence subsequent step(s) and are preferentially lost during recombination process.

palindrome; recombination; yeast; plasmid integration

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