engleski

Lipid peroxidation in dogs naturally infected with Babesia canis canis

Possible role of oxidative stress in the pathogenesis of infections caused by parasites is active area of research in recent years. The aim of this study was to assess changes in activity concentration of malondialdehyde (MDA), commonly used biomarker for assessing lipid peroxidation, in dogs infected with Babesia canis canis, after therapy with imidocarb dipropionate and to compare them to MDA concentration prior to treatment and to healthy controls. The study was conducted on a sample of 30 dogs suffering from babesiosis and 25 healthy dogs (control group). Blood was taken before treatment, first day (all dogs) and on second, third, and seventh day of research (affected dogs and ten healthy dogs). Application of imidocarb diproprionate in ten healthy dogs did not affect the values of MDA therefore in our study all healthy dogs were used as controls. On the first day of research the MDA concentration was significantly increased in dogs with babesiosis (P<0.001) in comparison to the control group. On seventh day MDA decreased, but was still significantly higher compared to the control group (P<0.001). Concentration of MDA on the first day was significantly increased in dogs with uncomplicated (P<0.001) and complicated babesiosis (P<0.001) compared to controls. On seventh day the concentration of MDA was significantly higher in the group of dogs with complicated babesiosis (P=0.003) than in uncomplicated group. Furthermore, dogs in multiple organ dysfunction syndrome group had significantly increased MDA concentration compared to dogs in uncomplicated (P=0.006) and single complication group (P=0.025). There was a significant positive correlation between MDA concentration and the outcome of the disease (Tau-b 0.309 ; P=0.005). The present study concludes that, on seventh day, increased lipid peroxidation is still present in the affected group of dogs. The results indicate that oxidative stress could have a possible causative role in the clinical severity of the disease.

oxidative stress; malondialdehyde; uncomplicated form of babesiosis; complicated form of babesiosis

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