STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA (CROSBI ID 621003)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gornik, Olga ; Knežević, Ana ; Pučić, Maja ; Redžić, Irma ; Wagner, Jasenka ; Lauc, Gordan
engleski
STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA
Glycan heterogeneity was shown to be associated with numerous diseases and glycan analysis has a great diagnostic potential. Recently, we reported high biological variability of human plasma N-glycome at the level of population. The observed variations were larger than changes reported to be associated with some diseases ; thus, it was of great importance to examine the temporal constancy of human N-glycome before glycosylation changes could be routinely analyzed in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during 5 days. N-Linked glycans, released from plasma proteins, were separated using hydrophilic interaction high performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of N-glycome in a single individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1 were observed. The average coefficient of variation was 5.6%. These variations were comparable to those observed when analytical procedure was tested for its precision. Good stability of plasma N-glycome in healthy individuals implies that glycosylation is under significant genetic control. Changes observed in glycan profiles are consequence of environmental influences and physiologic responses and therefore have a significant diagnostic potential.
glycans; stability
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Podaci o prilogu
2009.
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objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
The Sixth ISABS Conference on human genome project based applications in Forensic Science, Anthropology and Individualized medicine
poster
01.06.2009-05.06.2009
Split, Hrvatska