engleski

The effect of the zeolite clinoptilolite on serum chemistry and hematopoesis in mice

Zeolites are natural or synthetic crystalline alumosilicates with ion exchange properties. Supplied in fodder, they promote biomass production and animal health. Our aim was to asses the effects of the natural zeolite, clinoptilolite on hematopoesis, serum electrolytes, and essential biochemical indicators of kidney and liver function in normal and in mice with transplanted tumors. Two preparations differing in particle size were tested: a powderized form obtained by countercurrent mechanical treatment of the clinoptilolite (MTCp) and normally ground clinoptilolite (NGCp). Young adult mice were supplied with food containing 12.5%, 25% and 50% clinoptiloilte powder. Control animals received the same food ration without the clinoptilolite. After 10, 20, 30 and 40 days, six animals from each group were exsanguinated as to obtain blood for hematological and serum for biochemical measurements as well as to collect femoral bone marrow for determination of hematopoetic activity. Clinoptilolite ingestion was well tolerated, as judged by comparable body masses of control and treated mice. A 20% increase of potassiuim level was detected in mice receiving the zeolite-rich diet, without other changes in serum chemistry. Erythrocyre, hemoglobin and platelet levels in peripheral blood were not materially affected. NGCp caused leukocytosis, with concomitant decline of the GM-CFU content in the bone marrow. The MTCp preparation caused similar, albeit less pronounced changes. Mice having tranplanted mammary carcinoma in the terminal stage showed increased potassium and decreased sodium and chloride levels, severe anemia and leukocytosis, decreased bone marrow cellularity and diminshed content of hematopoietic progenitor cells in the marrow. The clinoptilolite preparations ameliorated the sodium and chloride decline, whereas the effects on hematopoesis were erratic. In conclusin, clinoptiloilte was well tolerated and did not cause major metabolic or hematologic disturbances in mice.

zeolite; clinoptilolite; mouse; serum chemistry; hematopoesis

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