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Experimental congenital listeriosis: a murine model (CROSBI ID 482518)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Abram, Maja ; Vučković, Darinka ; Dorić, Miljenko ; Wraber, Branka ; Hof, Herbert ; Schluter, Dirk ; Deckert, Martina Experimental congenital listeriosis: a murine model // Proceedings of ISOPOL XIV / Hof, Herbert (ur.). Mannheim: Universitatsklinikum, Insti. fur Med. Mikrobiol. unu Hygiene, 2001. str. 95-x

Podaci o odgovornosti

Abram, Maja ; Vučković, Darinka ; Dorić, Miljenko ; Wraber, Branka ; Hof, Herbert ; Schluter, Dirk ; Deckert, Martina

engleski

Experimental congenital listeriosis: a murine model

Problem: Listeria monocytogenes may cause severe infections in pregnancy with detrimental effects on the fetus. Since normal pregnancy is associated with a Th2 cytokine pattern in order to prevent rejection of the semiallogeneic fetus, an ongoing infection which elicits a Th1 response may be detrimental for pregnancy. Our work intends to study prenatal listeriosis of both fetus and the pregnant female. Method: Balb/c mice were infected intravenously on various days of pregnancy, and sacrificed 1, 2, 3 and 6 days thereafter. Bacterial clearance was monitored by plating of tissue homogenates. Immunohistochemistry was applied to topographically analyse the leukocytic infiltrates and their topographical relation to the bacteria in the utero-placental tissue. A panel of Th1 and Th2 cytokines was quantified by enzyme-immunoassay in maternal blood and also analysed in placenta and maternal liver by RT-PCR. Uninfected pregnant mice and Listeria monocytogenes infected non-pregnant Balb/c mice served as controls. Results: Gestating Balb/c mice showed an increased susceptibility to primary L. monocytogenes infection with a significantly increased rate of abortion. Clearance of bacteria and duration of listeriosis were prolonged. Histopathology revealed more severe inflammation in pregnant animals as compared to virgin controls. In the placentas of infected animals Listeriae were detected in clusters. Both serum and liver of pregnant animals exhibited markedly lower mRNA and protein level of the proinflammatory cytokines TNF-á and IFN-ă as compared to L. monocytogenes-infected non-pregnant mice. Conclusion: The reduced levels of pro-inflammatory, anti-listericidal cytokines may contribute to the aggravated disease and the poor outcome of congenital listeriosis. On the other hand, even the reduced production of Th1 cytokines may be incompatible with a successful pregnancy and enhance abortion.

Listeria mnocytogenes; pregnancy; cytokines

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Podaci o prilogu

95-x.

2001.

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objavljeno

Podaci o matičnoj publikaciji

Proceedings of ISOPOL XIV

Hof, Herbert

Mannheim: Universitatsklinikum, Insti. fur Med. Mikrobiol. unu Hygiene

Podaci o skupu

International Symposium on Problems of Listeria

pozvano predavanje

13.05.2001-16.05.2001

Mannheim, Njemačka

Povezanost rada

Kliničke medicinske znanosti