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Accumulation of defective interfering viral particles in only a few passages in Vero cells attenuates mumps virus neurovirulence (CROSBI ID 216979)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Šantak, Maja ; Markušić, Maja ; Lang Balija, Maja ; Keč Kopač, Sandra ; Jug, Renata ; Örvell, Claes ; Tomac, Jelena ; Forčić, Dubravko Accumulation of defective interfering viral particles in only a few passages in Vero cells attenuates mumps virus neurovirulence // Microbes and infection, 17 (2015), 3; 228-236. doi: 10.1016/j.micinf.2014.11.006

Podaci o odgovornosti

Šantak, Maja ; Markušić, Maja ; Lang Balija, Maja ; Keč Kopač, Sandra ; Jug, Renata ; Örvell, Claes ; Tomac, Jelena ; Forčić, Dubravko

engleski

Accumulation of defective interfering viral particles in only a few passages in Vero cells attenuates mumps virus neurovirulence

Immunization programs have implemented live attenuated mumps vaccines which reduced mumps incidence ≥97%. Some of the vaccine strains were abandoned due to unwanted side effects and the genetic marker of attenuation has not been identified so far. Our hypothesis was that non-infectious viral particles, in particular defective interfering particles (DIPs), contribute to neuroattenuation. We showed that non-infectious particles of the mumps vaccine L-Zagreb attenuated neurovirulence of wild type mumps virus 9218/Zg98. Then, we attenuated recent wild type mumps virus MuVi/Zagreb.HRV/28.12 in Vero cells through 16 passages but already the fifth passage (p5) showed accumulation of DIPs and attenuated neurovirulence in a newborn rat model when compared to the second passage (p2). Sequence analysis of the p2 and p5 revealed a single mutation in the 5' untranslated region of the HN gene. Analysis of the expression level of the HN protein showed that this mutation does not affect the expression of the protein. We conclude that the passages of MuVi/Zagreb.HRV/28.12 in Vero cells for only three passages accumulated DIPs which attenuate neurovirulence. These findings reveal DIPs as a very promising and general neuroattenuating factor which should be considered in the rational design of the new mumps vaccine.

Attenuation; Defective interfering particles; Infectious virus; Mumps virus; Neurovirulence

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Podaci o izdanju

17 (3)

2015.

228-236

objavljeno

1286-4579

10.1016/j.micinf.2014.11.006

Povezanost rada

Javno zdravstvo i zdravstvena zaštita, Biotehnologija, Biologija

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