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izvor podataka: crosbi

Sensing of Double-Stranded DNA/RNA Secondary Structures by Water Soluble Homochiral Perylene Bisimide Dyes (CROSBI ID 217004)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Gershberg, Jana ; Radić Stojković, Marijana ; Škugor, Marko ; Tomić, Sanja ; Rehm, Thomas H. ; Rehm, Stephanie ; Saha-Möller, Chantu R. ; Piantanida, Ivo ; Würthner, Frank Sensing of Double-Stranded DNA/RNA Secondary Structures by Water Soluble Homochiral Perylene Bisimide Dyes // Chemistry : a European journal, 21 (2015), 21; 7886-7895. doi: 10.1002/chem.201500184

Podaci o odgovornosti

Gershberg, Jana ; Radić Stojković, Marijana ; Škugor, Marko ; Tomić, Sanja ; Rehm, Thomas H. ; Rehm, Stephanie ; Saha-Möller, Chantu R. ; Piantanida, Ivo ; Würthner, Frank

engleski

Sensing of Double-Stranded DNA/RNA Secondary Structures by Water Soluble Homochiral Perylene Bisimide Dyes

A broad series of homochiral perylene bisimide (PBI) dyes were synthesized that are appended with amino acids and cationic side chains at the imide positions. Self-assembly behavior of these ionic PBIs has been studied in aqueous media by UV/Vis spectroscopy, revealing formation of excitonically coupled H-type aggregates. The interactions of these ionic PBIs with different ds-DNA and ds-RNA have been explored by thermal denaturation, fluorimetric titration and circular dichroism (CD) experiments. These PBIs strongly stabilized ds-DNA/RNA against thermal denaturation as revealed by high melting temperatures of the formed PBI/polynucleotide complexes. Fluorimetric titrations showed that these PBIs bind to ds-DNA/RNA with high binding constants depending on the number of the positive charges in the side chains. Thus, spermine-containing PBIs with six positive charges each showed higher binding constants (log Ks = 9.2 – 9.8) than their dioxa analogues (log Ks = 6.5 – 7.9) having two positive charges each. Induced circular dichroism (ICD) of PBI assemblies created within DNA/RNA grooves was observed. These ICD profiles are strongly dependent on the steric demand of the chiral substituents of the amino acid units and the secondary structure of the DNA or RNA. The observed ICD effects can be explained by non-covalent binding of excitonically coupled PBI dimer aggregates into the minor groove of DNA and major groove of RNA which is further supported by molecular modeling studies.

chiral perylene bisimide; DNA/RNA recognition; ionic self-assembly; groove binding; induced circular dichroism

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Podaci o izdanju

21 (21)

2015.

7886-7895

objavljeno

0947-6539

1521-3765

10.1002/chem.201500184

Povezanost rada

Fizika, Kemija

Poveznice
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