engleski

The occurrence and maintenance of Leptospira serovars Australis and Bratislava in domestic and wild animals in Croatia

In a 10-year period, from 2002 to 2011, 20157 sera samples and 984 kidneys of wild and domestic animals were collected and tested for leptospirosis in Laboratory for leptospirosis Veterinary Faculty University of Zagreb. Out of 19732 sera samples of horses 3876 (19.64%) had agglutinating antibodies against one or more Leptospira serovars. The highest seroprevalence in horses was found for the following serovars: sv Bratislava, sv Pomona and sv Australis. In wild boars, out of 215 samples 75 (34.88%) were positive and the most prevalent serovars were sv Australis, sv Grippotyphosa and sv Tarassovi. Out of 170 pig sera we found 66 (38.82%) positive animals. The most prevalent serovars were sv Australis, sv Ballum and sv Saxkoebing. In red foxes, out of 59 sera samples 34 (57.60%) were positive on leptospirosis. The highest titre we found for sv Australis, sv Sejroe, sv Saxkoebing and sv Grippotyphosa. Out of 151 sera samples of dogs 26 (17.22%) had antibodies for Leptospira serovars. The highest seroprevalence were for sv Pomona, sv Grippotyphosa, sv Australis and sv Icterohaemorrhagiae. Out of 262 kidney samples taken from the yellow-necked field mouse (Apodemus flavicollis) Leptospirae were isolated from 32 (12.21%) samples including 13 (40.63%) isolates of L. interrogans, serogorup Australis, sv Bratislava ; five (15.62%) isolates of L. borgpeterseni, serogoup Sejroe, sv Saxkoebing ; four (12.5%) isolates of L. interrogans, serogroup Australis, sv Muenchen-FR. From 122 kidney samples of the long-tailed field mouse (Apodemus sylvaticus) 22 (18.03%) were positive for Leptospira. Most frequently isolated Leptospira were: seven (31.82%) isolates of L. interrogans, serogroup Australis, sv Bratislava ; six (27.27%) isolates of L. interrogans, serogroup Australis, undetermined serovar ; three (13.64%) isolates of L. interrogans, serogroup Australis, sv Muenchen-FR. Out of 96 kidney samples of the black-striped field mouse (Apodemus agrarius) from 29 (30.21%) we isolated Leptospira. Most frequent serovars were: L. kirschneri, serogroup Pomona, sv Mozdok in eight (27.59%) samples, L. kirschneri, serogroup Bataviae, sv Bataviae in two (6.9%) samples while 19 (65.52%) isolates are still undetermined. Considering results of our and previous investigations of leptospirosis in Croatia we can conclude that Leptospira serovars from serogroup Australis, sv Bratislava, sv Australis and sv Lora are maintained among wild life animal species. Results of this and our previous studies of leptospirosis in wild animal species in Croatia strongly support a conclusion that wild carnivores and omnivores like red fox, wild boar and brown bear could also be maintaining reservoir hosts for serovars from serogroup Australis.

Leptospira ; Australis ; Bratislava ; reservoirs ; Croatia

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