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Different F18-FDG uptake in mouse brain after normobaric hyperoxia treatment

ABSTRACT The free radical theory of ageing first described by Denham Harman strongly correlate ageing with level of macromolecules damage caused by reactive oxygen species (ROS). The model of oxidative stress induced by hyperoxia using pure oxygen (92-95%) is well known. Reactive oxygen species (ROS), superoxide anion and hydrogen peroxide can be produced from inhaled oxygen in mitochondria and cell cytoplasm. The response to oxidative stress is sex-related, with female animals being more resistant to oxidative damage than males. In addition females have lower levels of ROS in cell, higher activity of antioxidant enzymes and higher mitochondrial respiratory chain capacity, which results with lower oxidative damage of macromolecules. The inverse relationship between glucose consumption and oxidative stress in brain tissue was already described by C. Borras et al. In addition there is little or no data of different response to hyperoxia between sex and age in relation to glucose consumption This work will described the effect of hyperoxia on brain glucose consumption as a potential parameter of oxidative stress. Our previous data already shown that after hyperoxia exposure female mice have higher survival rate in comparison to males. Ovariectomy diminished female resistance to hyperoxia oxidative damage, while estrogen implants in males increased their resistance to hyperoxia. Results of F18 FDG uptake demonstrated that older female mice have lower glucose consumption in the back brain i relation to young females. The same is not observed with males. In addition the effect of hyperoxia with pure oxygen (92-95%) on glucose consumption in beck brain is gender related with lower glucose consumption in female in relation to male animals. This data will additionally explain differences between age and sex in response to hyperoxia using brain glucose consumption status as marker of oxidative stress after hyperoxia treatment.

F18-FDG ; mouse ; brain ; normobaric ; hyperoxia

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