engleski

The amount of hydroxyapatite within the scaffold affects bone formation

Regenerative orthopedics seeks to establish a successful protocol for the healing of severe bone damage. The existing methods have a number of disadvantages and the goal of tissue engineering strategies is to develop a new scaffold that is biocompatible, biodegradable, non-immunogenic and has osteoinductive properties. For that purpose, we propose a new model of bone graft engineering using human mesenchymal stem cells (hMSCs) as a cell source, composite scaffold based on chitosan and hydroxyapatite (HA) and osteogenic media in combination with the perfusion force as a osteoinductive environment. The main aim of this study was to compare the influence of the amount of hydroxyapatite (HA) within the scaffolds on osteogenic differentiation of hMSCs in a U-CUP perfusion bioreactor (Cellec Biotek). We used and compared scaffolds with 0% HA, 10%, HA 30% and 50% HA. Bone grafts were analyzed after 14 and 21 days by histology and qPCR. Bone graft analysis after 14 and 21 days of culture showed that cells and connective tissue are present within the graft. qPCR analysis of bone markers, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and osteocalcin (OC), confirmed osteogenic differentiation of cells within the bone graft. We conclude that composite scaffold made of chitosan and 30% HA resulted in homogenous cell attachment and new tissue formation with the highest expression of osteogenic markers.

Chitosan; Hydroxyapatite; Scaffold; Bioreactor; Human mesenchymal stem cells (hMSCs); Osteogenic differentiation (qPCR

nije evidentirano