EXPRESSION OF WATER CHANNEL AQP1 IN PIG KIDNEYS IS GENDER-DEPENDENT (CROSBI ID 623927)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Brzica, Hrvoje ; Žura Žaja, Ivona ; Ljubojević, Marija ; Herak- Kramberger, Carol M. ; Del Vechio, Igor ; Milinković-Tur, Suzana ; Ćurković, Snježana ; Vuković, Snježana ; Špiranec, Katarina ; Bačić, Goran ; Maćešić, Nino ; Budinšćak, Zvonimir ; Sabolić, Ivan
engleski
EXPRESSION OF WATER CHANNEL AQP1 IN PIG KIDNEYS IS GENDER-DEPENDENT
Water channel 1 (AQP1), a small integral membrane protein expressed in the basolateral (BLM) and apical (AM) cell membranes of proximal tubule and thin descending limb of Henle, mediates ≥ 65% of water reabsorption in the mammalian kidneys. The expression of AQP1 has been extensively studied in various rat and human organs, while the corresponding data in pig are mostly restricted to the reproductive tract. The aim of this work was to determine whether the expression of AQP1 in pig kidneys is influenced by sex hormones. Swedish landrace pigs (10 of each sex) were either sham operated (5 males (M) and 5 female (F)) or gonadectomized (5 M and 5 F) at the age of 4 weeks and sacrificed at the age of 10 months. Kidney tissue samples were collected and tested for AQP1 protein expression by Western blotting of isolated membranes and by immunostaining of tissue cryosections using specific polyclonal antibodies. With both methods, the expression of AQP1 protein along the nephron was strongly gender-depend, being in sham operated F˃M. Castration had no effect, while ovariectomy downregulated the expression approximately to the levels in sham operated M. These data indicate that the expression of AQP1 in pig kidneys is stimulated by estrogens. This is in contrast to the situation in rats, where the renal AQP expression is male dominant due to androgen stimulation. Opposite gender- and sex hormone-driven AQP1 protein expression in rat and pig kidneys proves the existence of species differences regarding this water channel.
aquaporin 1; pig kidneys; gender dependent
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Podaci o prilogu
31-31.
2014.
objavljeno
Podaci o matičnoj publikaciji
Book of abstracts of the 2nd International Scientific Meeting of Anatomy and Physilogy Fundementals of medicine
Vilić, Marinko ; Lucić, Hrvoje
Zagreb: Veteriranrski fakultet Sveučilišta u Zagrebu
987-953-6062-98-0
Podaci o skupu
2nd International Scientific Meeting of Anatomy and Physilogy Fundementals of medicine
predavanje
16.06.2014-17.06.2014
Zagreb, Hrvatska