engleski

Serum levels of chemokines KC and IL-8 in dogs natural infected with Babesia canis canes

Canine babesiosis caused by Babesia canis canis is one of the commonest canine diseases in Croatia. It is known that systemic inflammatory syndrome (SIRS), which is a hallmark of babesiosis, is regulated by the host production of several proinflammatory and antiinflammatory cytokines that have been implicated as playing a critical role in the pathogenesis of the disease. In the course of larger research serum concentration of CXC chemokines keratinocyte chemoattractant (KC) and interleukine 8 (IL-8) was determined. The aim of the present study was to determinate serum chemokine concentration and to compare values between healthy and dogs with babesiosis. Additional aim was to determine if KC and IL-8 serum concentration is an important contributing factor in development of complicated babesiosis and does it affect the outcome of the disease. Serum chemokine activity was assessed by a canine- specific multiplex assay (CCYTO-90K, Millipore, Billerica, MA) with an automated analyzer (Luminex 200, Luminex Corporation, Austin, TX). Results are suggesting that higher serum concentration of KC-like is conected with severity of anemia, leukocytosis and developing of complicated type of babesiosis. IL-8 concentration positively correlates with granulocyte- lymphocyte ratio. In addition concentration of both chemokines was higher in dogs with complicated babesiosis. Therefore, concentration of observed chemokines could be considered as important factor in pathogenesis of canine babesiosis

KC; IL-8; inflammatory response; dog; babesiosis

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