engleski

Cytochrome P450 - CYP2D6 polymorphism in head and neck cancer patients

We investigated the possible association of drug metabolizing enzyme system CYP P450 CYP2D6 and its null alleles (CYP2D6*3, *4, *5, *6, *7, and *8) with incidence of tumors in patients having head and neck cancer (HNC). It is known that persons bearing two null alleles poorly metabolize some common drugs (Poor Metabolizer Phenotype – PM) as well as other foreign and carcinogenic substances. Persons with only one disrupted CYP2D6 gene (bearing one normal and one null allele) are considered to be Intermediate metabolizer phenotype (IM). We genotyped 145 controls, and 42 HNC patients by Multiplex Allele Specific PCR on whole blood DNA. Study results showed allelic frequencies for *3, *4 and *6 alleles (only alleles observed) in controls to be 1.4%, 11.0% and 1.0%, respectively ; among them we found 2.1% PMs and 22.8% IMs. In cancer patient’s group allelic frequencies for *3, *4 and *6 were 1.2%, 19.0% and 3, 6% respectively, and no other alleles were found ; among them we found 2.4% PMs and 42.9% IMs. Results of our study showed statistically significant difference for genotype frequencies (Chi-square ; p=0.025) and predicted phenotype (Chi-square ; p=0.034). IM phenotype showed to be responsible for increased risk to HNC (Odds ratio 2.6 ; 95%CI= 1.248 - 5.193). To confirm our preliminary findings, further study on a larger group is planned.

CYP2D6; head and neck cancer

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