Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Effects of herbaceous plant preparation of Cistanche on in vitro cytokine expression by rat osteoblasts (CROSBI ID 219110)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Caihong , Li ; Qing, Li ; Zhongjie, Liu ; Xiaoxu, Xing ; Dan, Tian ; Vrbanac, Zoran ; Smolec, Ozren, Stanin, Damir, Kos, Josip Effects of herbaceous plant preparation of Cistanche on in vitro cytokine expression by rat osteoblasts // Veterinarski arhiv, 85 (2015), 3; 335-345

Podaci o odgovornosti

Caihong , Li ; Qing, Li ; Zhongjie, Liu ; Xiaoxu, Xing ; Dan, Tian ; Vrbanac, Zoran ; Smolec, Ozren, Stanin, Damir, Kos, Josip

engleski

Effects of herbaceous plant preparation of Cistanche on in vitro cytokine expression by rat osteoblasts

Osteoblasts (OB) play an important role in bone remodeling thro ugh the release of cytokines such as o s t e o p r o t e g e r i n osteoprotegerin ( ( O P G ) , a r e c e p t o r a c t i v a t o r o f n u c l e a r f a c t o r k a p p a - B l i g a n d OPG), a receptor activator of nuclear factor kappa-B ligand (RA NKL) and osteopontin (OPN). The objective of our study was to evaluate the effects o f Chinese herb extracts of Cistanche (Cistanche deserticola ) on the in vitro expression of these cytokines in rat OB cell culture. The prim ary OBs were isolated from the rat skull by trypsin and collagenase digestion. The ce llular phenotype was determined by the Swiss dyeing method, alkaline phosphatase staining, Von Kossa stainin g, and Alizarin red staining. The gene expression of OPG, RANKL and OPN were analyzed by quantitative real-time P CR. All treatments with herbaceous plant extracts of Cistanche increased the gene expression of OPG and RANKL at 72 hours of culturing, especially at concentrations of 5×10 -2 mg/mL, which were signifi cantly different compared to the control cultures. Treatment signifi cantly reduced the expression of the OPG/RANKL ratio at 48 hou rs and the expression of OPN mRNA at 72 hours of culturing. The in vitro exposure of OB to the plant preparation of Cistanche promoted the gene expression of OPG and RANKL and reduced the gene expression of OPG/RANKL and OPN, and thus, could maintain the balance of bone formation and resorption during bo ne metabolism

OSTEOBLASTS; OSTEOPROTEGRIN; RANKL; RAT

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

85 (3)

2015.

335-345

objavljeno

0372-5480

Povezanost rada

nije evidentirano

Indeksiranost