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Molecular characterization, functional and phylogenetic analysis of paralogous SSB proteins from Streptomyces coelicolor

The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Mutational analysis of ssb genes indicated that ssbA is essential, whereas ssbB has a key role in chromosome segregation during sporulation. The two ssb genes were expressed differently, in accordance with their proposed biological roles. Localisation of fluorescently tagged SSB proteins was observed in the vegetative and aerial hyphae. Paralogous SSB proteins bind ssDNA with different affinity. Phylogenetic analysis of eubacterial SSBs indicated frequent duplication, loss of ssb genes and polyphyletic origin of well defined groups of SsbB proteins.

SSB; paralogous proteins; Streptomyces coelicolor; chromosome segregation; phylogeny

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