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Long Read RNA-seq Mapper (CROSBI ID 397074)

Ocjenski rad | diplomski rad

Marić, Josip Long Read RNA-seq Mapper / Šikić, Mile (mentor); Zagreb, Fakultet elektrotehnike i računarstva, . 2015

Podaci o odgovornosti

Marić, Josip

Šikić, Mile

engleski

Long Read RNA-seq Mapper

RNA sequencing is a technology that uses next generation sequencing tools for transriptome analysis. Bioinformatics tools used are used to analyze RNA sequences to reveal a snapshot of RNA presence and quantity from a genome at a given time which is important for interpreting the functional elements of the genome and understanding development of diseases. This thesis presents a tool built from the BBMap aligning method that is used in mapping long reads with high error rate to the reference genome. Improvements of the developed method are also presented and evaluated. Results are analyzes and compared to the BBMap tool. This paper explains the aligning method used by BBMap and shows that it can be recreated in such manner that it produces highly precise alignment results.

RNA sequencing; BBMap; precise alignment

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Podaci o izdanju

62

03.06.2015.

obranjeno

Podaci o ustanovi koja je dodijelila akademski stupanj

Fakultet elektrotehnike i računarstva

Zagreb

Povezanost rada

Računarstvo