engleski

First Report of NDM-1-Producing Acinetobacter guillouiae

ABSTRACT Since 2014 screening for metallo-β- lactamases (MBLs) of all Acinetobacter spp isolates by phenotypic methods and PCR has been implemented at the University Hospital Center Zagreb. The first MBL positive isolate was identified in Acinetobacter guiolloniae. The strain was isolated from drain of a newborn child hospitalized in a paediatric intensive care unit for heart malformation and identified as Acinetobacter guiolloniae with Maldi Tof automated system. The child was teated with meropenem and vancomycin prior to isolation of this strain. The strain was resistant to meropenem, imipenem, ceftazidime, cefotaxime, ceftriaxone, cefepime, gentamicin and ciprofloxacin and sulbactam/ampicillin, intermediate susceptible to piperacillin/tazobactam, and susceptible to colistin. Hodge test and combined disk test with EDTA were positive indicating the production of MBL. PCR and sequencing revealed blaOXA-58 and blaNDM-1 genes. This is the first report of NDM-1 in Acinetobacter spp in Croatia. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.

NDM-1 ; carbapenemases ; OXA-58 ; Acinetobacter guiollo

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