engleski

Phylogeography of Campanula fenestrellata s.l. (Campanulaceae) in the eastern Adriatic

Campanula fenestrellata Feer subsp. fenestrellata and C. fenestrellata Feer subsp. istriaca (Feer) Federov belong to the morphologically and phylogenetically well established amphi-Adriatic group Campanula ser. Garganicae Trinajstić. Those two endemic taxa are distributed on the north-eastern Adriatic islands and coast. C. fenestrellata subsp. istriaca is present from Istria to Jablanac (Velebit littoral) and on the northern Adriatic islands, while C. fenestrellata subsp. fenestrellata is distributed from Jablanac southwards to Krka river. Their taxonomic status varies and they have been treated as separated species by some authors. Although recently several molecular phylogenetic studies revealed poorly resolved relationships between taxa in the ser. Garganicae as well as conflicting relations between the nuclear ITS and chloroplast DNA sequences, the two C. fenestrellata taxa always formed a distinct lineage. In order to further study the relationships among C. fenestrellata s.l. populations and to gain insight into genetic basis of taxonomic separation established on morphology we examined amplified fragment length polymorphisms (AFLPs) as well as nuclear and plastid DNA sequences (ITS and trnL-F). The sequence data revealed a shallow structure and no distinction between two taxa was observed. The AFLPs separated populations geographically and STRUCTURE analysis resulted in an optimal partition with eight clusters. Admixed populations were evident in the geographic area around Jablanac and Karlobag where the two taxa overlap indicating the presence of hybridisation, while the northern and the southernmost populations were assigned to uniform separate clusters indicating the longer isolation of respective populations.

Campanula fenestrellata; phylogeography; taxonomy

nije evidentirano