engleski

Alpha, Beta and Gamma papillomaviruses prevalence in oral mucosa

Oral lesions have different etiology, although most of them can be largely attributed to environmental exposures, some of them can be linked to a persistent HPV infection. In this study, we wanted to elucidate in which proportion HPV infection is associated to particular oral lesions. In addition, we wanted to evaluate two types of consensus primers for HPV detection, MY09/11 and FAP primers, the first initially designed to amplify the majority of HPV types associated with mucosal (i.e. Alpha- papillomaviruses), while the second with cutaneous lesions (i.e. Beta- and Gamma- papillomaviruses). The study included 246 subjects with different oral lesions, and 73 subjects with apparently healthy oral mucosa (controls). Oral lesions were classified according to their surface morphology and clinical diagnosis. Oral epithelial cells were collected with the cytobrush. The presence of HPV DNA was evaluated by several consensus primer- directed polymerase chain reaction (PCR) and type-specific (TS) PCR for HPV types 6/11, 16, 18, 31, 33, 45, 52, and 58. Human beta-globin was also amplified to verify the suitability of the isolated DNA for PCR. The positivity of mucosal HPV types determined by MY09/11-PCR and TS-PCR was detected in 17.7% of oral lesions, significantly more than in apparently healthy mucosa (6.8%) (Mravak- Stipetic et al. PLoS One. 2013 ; 8(7):e69736). Mucosal high-risk HPV types were predominantly found in potentially malignant oral disorders, while benign proliferative lesions as well as healthy oral mucosa contained mainly undetermined HPV type. The positivity of cutaneous HPV types determined by FAP-PCR on a subgroup of 154 samples was 63.5%, which was significantly higher (Chi squate P = 0.0054) than with mucosal HPV primers in the same group (17.5%). In conclusion, we consider that in oral pathology HPV detection should be included in the regular diagnostic clinical protocol, and both mucosal and cutaneous HPV types should be investigated.

oral ; HPV

nije evidentirano