engleski

Phenolic constituents and antioxidant activity of ethanolic extracts of endemic plant species Centaurea ragusina L. (Asteraceae)

Centaurea ragusina L. (Asteraceae) is a Croatian endemic perennial plant. Like some other species from Centaurea genus, C. ragusina is also interesting as a source of different bioactive substances with potential medicinal or pharmaceutical applications. The goal of this work was to determine the levels of total phenolics and phenolic groups as well as antioxidant activity in 80% ethanolic extracts of C. ragusina cultivated in vitro (½MS 2.9 μM GA3 + 0.5 μM BA and ½MS 2.5 μM IBA), acclimated from culture conditions (½MS 2.5 μM IBA) and collected at natural habitats (Sustipan and Katalinić brig). The correlation coefficients were also identified between the levels of total phenolics and phenolic groups and antioxidant activity. The highest level of total phenols, hydroxycinnamic acids, flavonols and proanthocyanidins was observed in ethanol extracts of leaves collected at Katalinić brig while the highest value of flavonoids was detected in calli ethanolic extracts cultivated in vitro (2.9 μM GA3 + 0.5 μM BA). Significant antioxidant activity measured by DPPH, ABTS, NO and FRAP methods was recorded in almost all ethanolic extracts. A positive correlation was found between the levels of total phenolics, as well as various groups of phenolic compounds and antioxidant activity of the extracts.

Centaurea ragusina L.; phenols; antioxidant activity

Naziv projekta: Biološka aktivnost fitokemikalija biljne vrste Centaurea ragusina L.(BioFitoCen) Šifra projekta: HR.3.2.01-0290 Projekt sufinancira Europska unija: iz Europskog socijalnog fonda u sklopu programa Razvoj ljudskih potencijala 2007. – 2013.