engleski

Prevalence and resistance of Campylobacter spp. in retail poultry meat

We assessed Campylobacter spp. contamination of chicken neck skins and chest muscles from 60 retail chickens of different suppliers, collected in various markets in Rijeka during the year 2011. Bacteriological analyses were performed by conventional separation and identification procedures in accordance with ISO standards for Campylobacter. Campylobacters were detected in 19 (31.7%) of food samples with higher prevalence in the chicken neck skin samples than in chest muscles. The vast majority of poultry isolates belonged to C. jejuni (79%) and the rest were C. coli. At the same time 6444 stool samples from diarrhoeal patients were examined in the Laboratory for Diagnostics of Enteric Infections of the Teaching Institute of Public Health of the County Primorsko Goranska, Croatia and campylobacters were detected in 270 samples (4.2%). C. jejuni was confirmed in 89% and C. coli in 11% of human stool samples. All campylobacters were tested for the antimicrobial susceptibility to ampicillin, erythromycine, nalidixic acid, ciprofloxacin, gentamycine and tetracycline by disc diffusion method. Because of possible transmission of resistance through the food chain we expected to find similar resistance pattern in both poultry and human isolates. However, 55% of human and 33% of poultry isolates were resistant to ciprofloxacine and 53% of poultry meat vs. 9% of human isolates were resistant to tetracycline. None of the isolates (neither human nor poultry meat isolates) were resistant to gentamycine.

Campylobacter ; poultry ; antimicrobial resistance

Rad je izrađen uz potporu Sveučilišta u Rijeci: “Molecular mechanisms of bacterial pathogenesis and response to stress” (M.A., grant number 13.06.1.1.07. from the University of Rijeka, Croatia)