Assessment of primary DNA damage in organs of Wistar rats after acute exposure to different silver nanoparticles (CROSBI ID 628836)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Milić, Mirta ; Ljubojević, Marija ; Ćurlin, Marija ; Micek, Vedran ; Jurašin, Darija ; Pavičić, Ivan ; Gajović, Srećko ; Vinković Vrček, Ivana
engleski
Assessment of primary DNA damage in organs of Wistar rats after acute exposure to different silver nanoparticles
Silver nanoparticles (AgNPs), due to their biocidal activity, are commonly used as a coating or embedding agents for wound dressings, surgical instruments, implants and bone prostheses, odor-resistant textiles, deodorants, room sprays, water cleaners, laundry detergents, in food packaging and wall paints. The increasing use of nanosilver-containing products poses toxicological and environmental issues which need to be addressed. Here, we present results on primary DNA damage in blood, kidney and liver of 3 months old male and female Wistar rats after 30 min exposure to 1 mg/kg b.w. of silver nanoparticles (AgNPs). Comparison was done for effects of 3 types of AgNPs coated with polyvinylpyrrolidone (PVPAgNPs), metallothionein (METAgNPs), and bovine serum albumin (BSAAgNPs). Tail length, tail intensity (TI) and tail moment were measured by the alkaline comet assay. All experiments were performed in triplicate. In total, 300 comets per concentration were measured. Statistical analysis was performed for TI as recommended by OECD for in vivo genotoxicity testing of chemicals. In the female group, significantly higher DNA damage compared to controls was seen in blood and liver for all AgNPs types, while in kidney samples only BSAAgNPs treatment induced significantly higher TI. In male group, only liver showed significantly higher TI than controls for all three types of AgNPs. In male blood, only METAgNPs induced significant DNA damage compared to controls, while kidneys of males treated with PVPAgNPs and BSAAgNPs showed significantly lower TI values than controls. Since the physicochemical properties of AgNPs such as particle size, surface charge, surface functionalization, and dispersibility significantly influence the degree and actions of AgNPs, further research is required to assess the effects of these variables on their toxicity.
silver nanoparticles ; genotoxicity ; Wistra rat ; Comet assay
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Podaci o prilogu
26-26.
2015.
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objavljeno
Podaci o matičnoj publikaciji
Journal of trace elements in medicine and biology
Jurasović, Jasna ; Aschner, Michael
Amsterdam: Elsevier
0946-672X
Podaci o skupu
ISTERH Conference Recent Advances in Trace Element Research in Health and Disease (11 ; 2015)
poster
18.10.2015-22.10.2015
Srebreno, Hrvatska
Povezanost rada
Biologija, Javno zdravstvo i zdravstvena zaštita, Kemija