engleski

Antimicrobial activity of non-alcoholic propolis solution against common bovine mastitis pathogens

Bovine mastitis causes significant cost to dairy industry and is the main reason for antimicrobial use on dairy farms. Discarded milk following antimicrobial therapy of mastitis causes additional losses and may lead to presence of residues in milk. The aim of our study is to evaluate the in vitro antimicrobial activity of non-alchoholic solution of Croatian propolis against common bovine mastitis pathogens. In vitro antimicrobial activity of propolis was evaluated by agar-dilution method according to CLSI guidelines. Minimum inhibitory concentrations (MICs) were determined for approximately 100 isolates of common mastitis pathogens. Propolis showed good activity against Trueperella pyogenes, streptococci, Staphylococcus aureus and coagulase-negative staphylococci, with minimum inhibitory concentrations ranging from 8 to 64 mg/L. Significantly lower activity was determined against E. coli and Pseudomonas aeruginosa with MICs ≥5000 mg/L. Our results indicate that non- alcoholic propolis solution has good in vitro activity against gram- positive bacteria causing bovine mastitis. Further clinical trials are conducted to asses its in vivo efficacy for the intramammary treatment of mastitis.

mastitis ; cow ; propolis ; antimicrobial activity

nije evidentirano