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Animal models of psoriasis: antiinflammatory and antioxidant epigallocatehin gallate efficiency

We examined positive effects of epigallocatehin gallate (EGCG) on an animal model of psoriasis, in which the psoriasis was caused by di‐n‐ propyl disulphide (PPD) irritant during 5 days. Anti‐inflammatory and antioxidative activity of EGCG preparations were followed by various methods: cell numbers in abdominal cavity, functional activity of macrophages in peritoneal fluid, pathohistological analyses of skin, haematological and biochemical parameters of blood and antioxidative efficiency by reduced glutathione and lipid peroxidation. The results indicate that EGCG preparations reduce number of inflammatory cells in peritoneal fluid of mice. All haematological and biochemical parameters indicate that it falls within the reference range while cytokines' values in plasma indicate that PPD alone, or combined with EGCG, caused a higher level of antiinflammatory cytokines, especially granulocyte‐col ony stimulating factor (G‐CSF) and interleukins‐6 (IL‐6). Pathohistological analysis of skin samples indicates that a topical use of the irritant with EGCG causes significantly lower inflammatory reaction than PPD itself. The results of comet and micronucleus test show no genotoxicity induced by EGCG preparations in peripheral blood cells of mice. We conclude that EGCG preparations show antiinflammatory effect and their use could be applicable in a suppression and treatments of inflammatory process in psoriasis, without any toxical effects.

mouse models of psoriasis ; skin lesions ; thermography ; EGCG

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