engleski

Groups of genes epigenetically modified in cervical cancer

The relationship between oncogenic human papilloma viruses (HPVs) and cervical cancer development is well established. It is known that HPV infection is widespread but cervical cancer develops in only certain cases. The fact is that little is known about the secondary factors associated with progression from precancer lesions to cervical cancer. Silencing of gene expression through epigenetic change such as DNA methylation is one of the early events of carcinogenesis. Therefore, in this study, we established DNA methylation profiles for groups of cellular genes modified regarding to HPV infection and cervical diagnosis (HPV negative normal cervical scrapes, HPV positive cervical intraepithelial neoplasia grade 3 [CIN3], and HPV positive squamous cell carcinoma). The Illumina Infinium HumanMethylation450 BeadChip method identifies genome-wide DNA methylation changes in CpG islands, CpG shores and shelves. Our findings showed an extensive differential methylation signature in cervical cancer compared with the CIN3 or normal cervical tissues. The identified group of genes that is epigenetically deregulated by hypermethylation in cervical cancer compared with the CIN3 or normal cervix includes genes associated with the cellular machinery mechanisms, such as intracellular signaling and transcription. The other group of genes was extensive hypomethylated in cancer tissues and those genes are included in the immune system response. Therefore, altered methylation profiles could be a good predictor of cancer and precursor lesions. Moreover, DNA methylation profiles can provide an insight into the host cell gene status and changes when these cells are infected with HPV.

HPV ; cervical cancer ; methylation ; gene

nije evidentirano