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Pregled bibliografske jedinice broj: 790355

Časopis

Autori: Knezović, Ana; Osmanović-Barilar, Jelena; Ćurlin, Marija; Hof, Patrick R.; Šimić, Goran; Riederer, Peter; Šalković-Petrišić, Melita
Naslov: Staging of cognitive deficits and neuropathological and ultrastructural changes in streptozotocin-induced rat model of Alzheimer's disease
Izvornik: Journal of neural transmission (0300-9564) 122 (2015), 4; 577-592
Vrsta rada: članak
Ključne riječi: Alzheimer’s disease; Streptozotocin; Amyloid protein; Tau protein; Lysosomes; Cognitive decline
Sažetak:
Sporadic Alzheimer's disease (sAD) is the most common form of dementia. Rats injected intracerebroventricularly with streptozotocin (STZ-icv) develop insulin-resistant brain state and represent a non-transgenic sAD model with a number of AD-like cognitive and neurochemical features. We explored cognitive, structural and ultrastructural changes in the brain of the STZ-icv rat model over a course of 9 months. Cognitive functions were measured in the STZ- icv- (0.3, 1 and 3 mg/kg) and age-matched control rats by passive avoidance test. Structural changes were assessed by Nissl and Bielschowsky silver staining. Immunohistochemistry and electron microscopy analysis were used to detect amyloid β- (Aβ(1- 42)) and hyperphosphorylated tau (AT8) accumulation and ultrastructural changes in the brain. Memory decline was time- (≤3 months/acute, ≥3 months/progressive) and STZ- icv dose-dependent. Morphological changes were manifested as thinning of parietal cortex (≥1 month) and corpus callosum (9 months), and were more pronounced in the 3 mg/kg STZ group. Early neurofibrillary changes (AT8) were detected from 1 month onward in the neocortex, and progressed after 3 months to the hippocampus. Intracellular Aβ(1-42) accumulation was found in the neocortex at 3 months following STZ-icv treatment, while diffuse Aβ(1-42)-positive plaque-like formations were found after 6 months in the neocortex and hippocampus. Ultrastructural changes revealed enlargement of Golgi apparatus, pyknotic nuclei, and time- dependent increase in lysosome size, number, and density. Our data provide a staging of cognitive, structural/ultrastructural, and neuropathological markers in the STZ-icv rat model that in many aspects seems to be generally comparable to stages seen in human sAD.
Projekt / tema: HRZZ-09/16, IP-2014-09-9730
Izvorni jezik: ENG
Rad je citiran u
bazama podataka:
Web of Science: Science Citation
Current Contents Citation Index
Scopus
MEDLINE
Web of Science: Science Citation Index Expanded
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://link.springer.com/article/10.1007%2Fs00702-015-1394-4
Broj citata:
Altmetric:
DOI: 10.1007/s00702-015-1394-4
URL cjelovitog teksta:
Google Scholar: Staging of cognitive deficits and neuropathological and ultrastructural changes in streptozotocin-induced rat model of Alzheimer's disease
Upisao u CROSBI: Goran Šimić (gsimic@hiim.hr), 16. Pro. 2015. u 12:10 sati



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