A case of maternal-foetal chimerism identified during routine histocompatibility testing for hematopoietic stem cell transplantation (CROSBI ID 223733)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Grubić, Zorana ; Štingl, Katarina ; Kelečić, Jadranka ; Batinić, Drago ; Dubravčić, Klara ; Žunec, Renata
engleski
A case of maternal-foetal chimerism identified during routine histocompatibility testing for hematopoietic stem cell transplantation
This report describes a case of maternal–foetal chimerism identified in a boy diagnosed with SCID, who underwent HLA testing in preparation for HSCT. The first analysis was carried out on DNA from peripheral blood and included HLA-A, HLA-B, HLA- DRB1 typing using PCR-SSO. The patient’s HLA-B typing results were noninterpretable. All samples were retyped for HLA-B using PCR-SSP, again resulting in noninterpretable typing of patient’s HLA-B. In both cases, several weak positive probes/reactions interfered with the interpretation when using commercial software. Next round of HLA typing, using PCR-SSP and PCR- SSO methods, included the patient’s bone marrow sample and HLA-C locus, but interpretation was again not possible. The PCR-STR analysis performed on both peripheral blood and bone marrow samples revealed seven STRs for which two maternal and one paternal allele were detected. Retrospective manual interpretation of HLA-B and HLA-C typing revealed that weak positive reactions were indeed owed to paternal HLA-B and HLA-C alleles and that the patient had both maternal and one paternal allele. Retyping of HLA-B and HLA-C loci and STR analysis on the patient’s buccal cells sample revealed the expected one maternal/one paternal allele pattern. In summary, the combination of several different typing methods and manual interpretation were necessary to obtain the patient’s HLA typing results.
Chimerism; HLA; STR; Hematopoietic stem cell transplantation
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
Povezanost rada
Kliničke medicinske znanosti