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Charactrization of photosynthetic picoeukaryotes from the Southern Adriatic Pit (CROSBI ID 631950)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Mejdandžić, Maja ; Orlić, Sandi ; Bosak, Sunčica ; Bošnjak, Ivana ; Cetinić, Ivona ; Peharec Štefanić, Petra ; Sesar, Tihana ; Gašparović, Blaženka ; Ljubešić, Zrinka Charactrization of photosynthetic picoeukaryotes from the Southern Adriatic Pit // Abstracts of papers presented at the EMBO | EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes / Nürnberger, Tim (ur.). Heidelberg: Mera Druck GmbH, Sandhausen, Germany, 2016. str. 170-170

Podaci o odgovornosti

Mejdandžić, Maja ; Orlić, Sandi ; Bosak, Sunčica ; Bošnjak, Ivana ; Cetinić, Ivona ; Peharec Štefanić, Petra ; Sesar, Tihana ; Gašparović, Blaženka ; Ljubešić, Zrinka

engleski

Charactrization of photosynthetic picoeukaryotes from the Southern Adriatic Pit

Photosynthetic picoeukaryotes with cell size less than 3 μm are widely distributed in the world oceans and play critical role in oceanic primary production. In recent years, the composition and abundance of marine picoeukaryotes have been investigated using several approaches: morphological (LM, TEM) and chemical studies, flow-cytometry studies, chemotaxonomic and molecular studies. Adriatic Sea, the northern most part of Mediterranean is semi-enclosed oligotrophic basin where picophytoplankton is of an extreme importance for the functionality of the ecosystem. Until recently, our knowledge about phytoplankton community in Adriatic was limited to micro- and nanophytoplankton, while pico-fraction except of bacteria was neglected. During BIOTA cruise in February/March 2015, along two transects in Southern Adriatic pit, several clones of picoeukaryotes were isolated, cultivated and analysed using imaging, chemical and molecular methods. Photosynthetic picoeukaryotes were isolated from stations where flow cytometry analysis revealed their high abundances at greater depths (>280 m) well below photosynthesis active irradiance (PAR) values. Photosynthetic picoeukaryote plastid 16S rRNA gene was amplified with one set of primers - PLA491F/OXY1313R. Sequence and BLAST analysis of 4 monoclonal strains revealed 99-100% similarity with genera Nannochloris and Picochlorum, and some uncultured eukaryote clones. Lipid composition demonstrated clear domination of glycolipid sulfoquinovosyldiacylglycerol, on average 34% among all lipid classes. Combination of morphological, chemical, ecological and phylogenetic data allowed us to characterise Nannochloris-like microalgae from Southern Adriatic Pit.

photosynthetic picoeukaryotes ; Picoclorum ; Nannochloris ; Southern Adriatic Pit

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Podaci o prilogu

170-170.

2016.

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objavljeno

Podaci o matičnoj publikaciji

Abstracts of papers presented at the EMBO | EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes

Nürnberger, Tim

Heidelberg: Mera Druck GmbH, Sandhausen, Germany

Podaci o skupu

EMBO | EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes

poster

26.01.2016-29.01.2016

Heidelberg, Njemačka

Povezanost rada

Fizika, Kemija, Biologija