engleski

Synthesis of anthrose lipidic derivative as mimic of B. anthracis BclA glycoprotein for use in

The surfaces of Bacillus anthracis endospores expose anthrose-containing oligosaccharides, which have been considered for use as a target for specific detection of the spores. In this direction, we have developed an efficient and straightforward synthetic strategy toward anthrose lipidic derivate tetradecyl 4, 6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methyl- β- D -glucopyranoside 16 as a model target for B.anthracis spores. The ability of the prepared anthrose and glucose (for control purposes) lipidic derivatives to display on a multiwell plate was demonstrated by a colorimetric phenol-sulfuric acid assay and their potential utility in multiwell binding assays was assessed usingfluorescein-labeledconcanavalinA(ConA-FITC)andAleuria aurantia (AAL-FITC).

Anthrax ; anthrose ; binding ; glucose ; synthesis

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