Pathogenesis of murine cytomegalovirus infection in neonatal mice (CROSBI ID 465161)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Pernjak-Pugel, Ester ; Tomac, Jelena ; Trgovcich, Joanne ; Jonjić, Stipan
engleski
Pathogenesis of murine cytomegalovirus infection in neonatal mice
Cytomegalovirus (CMV) infections are a major source of morbidity and mortality in congenitally and perinatally-infected infants. Despite the extensive characterization of clinical disease induced by human CMV in neonatal infections of humans, fundamental questions regarding the pathogenesis of disease remain unanswered. For example, which tissues support virus replication and what is the nature of the tissue damage? What is the role of the immune response and cytokines in protection from or exacerbation of disease? Also, which viral determinants are essential to virulence and how does age at acquisition influence virulence? To begin to address these issues, we have established a model of disease in neonatal mice using both virulent and attenuated strains of murine CMV. Our approach to the study of MCMV pathogenesis in newborns was a comparative one, in which infection with tissue culture-derived virus was compared with an attenuated recombinant mutant lacking the fcr gene product. Investigation of MCMV pathogenesis in neonatal mice revealed several important aspects of disease. First, neonatal infection with WT virus follows a virulent disease course involving multiple tissues and organs. Absence of the fcr gene product, however, results in significant attenuation of disease course. Second, virus replication and damage in the brain and meninges was a primary feature of infection and was unique to newborn mice. Third, this study implicates immunopathological mechanisms of disease both in the CNS (neurovirulence), and in peripheral tissues and organs, especially the heart and connective tissues. In addition to morphological evidence supporting immunopathology in neonatal infections, the dramatic induction of TNF-a associated with the virulent WT virus also supports an immunopathological condition occurring in these mice.
cytomegalovirus
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Podaci o prilogu
P23-P23-x.
1997.
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objavljeno
Podaci o matičnoj publikaciji
Annual meeting of the Croatian Immunological Society, Periodicum biologorum 99 (1997), suppl. 2
Vitale, Branko
Zagreb: Hrvatsko prirodoslovno društvo
Podaci o skupu
Annual meeting of the Croatian Immunological Society 1997
pozvano predavanje
06.11.1997-07.11.1997
Zagreb, Hrvatska