Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

Effect of acute inflammation induced by lipopolysaccharide on Fas-mediated hepatocyte apoptosis in mice (CROSBI ID 634218)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Kelava, Tomislav ; Markotić, Antonio ; Ćavar, Ivan ; Turčić, Petra ; Šućur, Alan ; Ivčević ; Flegar, Darja ; Grčević, Danka Effect of acute inflammation induced by lipopolysaccharide on Fas-mediated hepatocyte apoptosis in mice. 2015

Podaci o odgovornosti

Kelava, Tomislav ; Markotić, Antonio ; Ćavar, Ivan ; Turčić, Petra ; Šućur, Alan ; Ivčević ; Flegar, Darja ; Grčević, Danka

engleski

Effect of acute inflammation induced by lipopolysaccharide on Fas-mediated hepatocyte apoptosis in mice

Fas/Fas ligand (FasL) apoptotic pathway is involved in the pathogenesis of various liver diseases. The exact effects of inflammation on the liver apoptotic processes are still not well understood. We investigated the effect of acute inflammatory response on Fas/FasL-mediated hepatocyte apoptosis using a model of lipopolysaccharide (LPS)-induced acute inflammation. Male C57BL/6 mice received intraperitoneal injection of LPS (0.1 μg/g) while the control group of animals received the vehicle (sterile saline). After 2 hours both groups were treated with anti-Fas activating antibody (0.25 μg/g, intravenously). Mice were sacrificed after additional 6 hours and plasma (ALT, AST) and liver (pathohistology, caspase activity, qPCR) were harvested. Mice pre-treated with LPS had lower levels of ALT (median (IQR) ; 82 (32-182) vs 3709 (1429 - 5922) U/L, p=0.02) and AST 151.5 (96-256) vs 3137 (1378-5389) U/L, p=0.02) in plasma, lower number of apoptotic cells on pathohistological analysis and lower caspase 8 activity than mice in the group receiving only anti-Fas treatment. LPS alone had no effect on aminotransferase levels and caspase 8 activity, while it increased expression of inflammatory mediators TNF-alpha, IL-1 and IL-6 in hematopoietic liver cells as well as the expression of Fas and antiapoptotic CFLAR and Bcl2l1 in hepatocytes. Acute inflammation induced by LPS protects hepatocytes from Fas/FasL-mediated apoptosis by acting on Fas-apoptotic pathway upstream of caspase 8 activation. We intend to define protective mechanism more precisely by investigating effects of LPS on expression pattern of broader spectrum of pro- and anti-apoptotic molecules at various time points following LPS treatment

apoptosis. liver injury; lipopolysaccharide; inflammation

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

2015.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

Podaci o skupu

4th European Congress of Immunology - ECI 2015

poster

06.09.2015-09.09.2015

Beč, Austrija

Povezanost rada

Temeljne medicinske znanosti