engleski

Exploring the stability of IgG and plasma glycosylation within healthy individuals

To explore the stability of IgG and Plasma glycosylation within healthy individuals as precisely as possible it is necessary to derive an experimental design that will take into account variability between individuals, together with the experimental variability of the procedures used for the experiments. Exploring the experimental variability is a tedious task but extremely important. A recommended solution is to use replicates together with the expert knowledge about possible pitfalls in the experimental procedure. This leads to an experimental design where multiple plates and conditions should be used. Variability between individuals can be incorporated into the experimental design by taking into account possible sources of variability within a population. Previous research has found traits like age and gender to be of utter importance together with the possibility of a high influence from the women menstrual cycle. This poster will present the results obtained from such an experiment where the experimental sources of variation were different analysts doing the sample preparation, time between batches of experiments and machine resolution ; while sampling of a population was based on age, gender and menstrual cycle. Ten people in total entered the experiment, blood was sampled three times during 10 days and everything was done in triplicates within a plate, with 6 plates for IgG and 6 plates for Plasma samples.

stability; igg; plasma; glycosylation

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