Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N- glycosylation of secreted glycoproteins (CROSBI ID 228561)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Klasić, Marija ; Krištić, Jasminka ; Korać, Petra ; Horvat, Tomislav ; Markulin, Dora ; Vojta, Aleksandar ; Reiding, Karli R. ; Wuhrer, Manfred ; Lauc, Gordan ; Zoldoš, Vlatka. DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N- glycosylation of secreted glycoproteins // Scientific reports, 6 (2016), 24363, 0. doi: 10.1038/srep24363

Podaci o odgovornosti

Klasić, Marija ; Krištić, Jasminka ; Korać, Petra ; Horvat, Tomislav ; Markulin, Dora ; Vojta, Aleksandar ; Reiding, Karli R. ; Wuhrer, Manfred ; Lauc, Gordan ; Zoldoš, Vlatka.

engleski

DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N- glycosylation of secreted glycoproteins

Changes in N-glycosylation of plasma proteins are observed in many types of cancer, nevertheless, few studies suggest the exact mechanism involved in aberrant protein glycosylation. Here we studied the impact of DNA methylation on the N-glycome in the secretome of the HepG2 cell line derived from hepatocellular carcinoma (HCC). Since the majority of plasma glycoproteins originate from the liver, the HepG2 cells represent a good model for glycosylation changes in HCC that are detectable in blood, which is an easily accessible analytic material in a clinical setting. Two different concentrations of 5-aza-2'-deoxycytidine (5- aza-2dC) differentially affected global genome methylation and induced different glycan changes. Around twenty percent of 84 glyco-genes analysed changed expression level after the 5-aza-2dC treatment as a result of global genome hypomethylation. A correlation study between the changes in glyco-gene expression and the HepG2 glycosylation profile suggests that the MGAT3 gene might be responsible for the glycan changes consistently induced by both doses of 5-aza- 2dC. Core-fucosylated tetra-antennary structures were decreased in quantity likely as a result of hypomethylated MGAT3 gene promoter followed by increased expression of this gene.

DNA ; methylation ; MGAT3 gene ; glycosylation

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

6

2016.

24363

0

objavljeno

2045-2322

10.1038/srep24363

Povezanost rada

Biologija

Poveznice
Indeksiranost